食品工业科技Issue(4)：370-372,3.
高效液相色谱法测定刺玫果中槲皮素的含量
Determination of quercetin in Rosa davurica.Pall by HPLC
摘要
Abstract
Objective:To develop a method for the determination of quercetin in Rosa davurica Pall. Method: HPLC analysis method was applied using CH3OH-0.2％ N3PO4 as gradient elution mobile phase with Shim-pack VP-ODS C18 ( 4.6mm × 150mm, 5μm ) column. The flow rate, injection volume of the sample and the column temperature were 1.OmL/min,20μL,40℃ respectively and the UV detection wave-length was set at 370nm. Results:There was good linear relationship between integral value of peak area and quercetin in the range of 0.05166～0.82656μg( r = 0.9999).The average recovery of quercetin was 97.38％ with the RSD of 1.79％.Conclusion: The determination of quercetin in Rosa davurica. Pall with HPLC method was studied in detail. The method was convenient and accurate which can be used for the quality control of Rosa davurica. Pall.关键词
刺玫果/槲皮素/高效液相色谱法Key words
Rosa davurica. Pall/ quercetin/ HPLC分类
轻工纺织引用本文复制引用
王晓林..高效液相色谱法测定刺玫果中槲皮素的含量[J].食品工业科技,2011,(4):370-372,3.基金项目
吉林省教育厅重点计划项目(吉教科合字[2006]第122号). （吉教科合字[2006]第122号）
