食品工业科技Issue(4)：397-399,404,4.
山黧豆中还原型谷胱甘肽的荧光检测
Determination of reduced glutathione in Lathyrus sativus L.by fluorometric method
摘要
Abstract
A method for measurement of reduced glutathione(GSH) in grass pea( Lathyrus sativus L)tissues was developed,with use of o-phthalaldehyde(OPA) as a fluorescent reagent. By estimating the effects of pH, OPA contents and reaction time on the formation of fluorescent products at room temperature, the optimal assay conditions were achieved,including the excitation and emission wavelengths of 347nm and 425nm, respectively,pH8.0,0.30mg · mL-1OPA and a 20-min reaction time. The fluorescence intensity was linear with the concentration of GSH in the range of 0.05～0.80μg · mL-1 ,with regression equation y = 1084x +63.148 and correlation coefficient 0.9990.The method present was rapid,simple, sensitive and accurate,and more suitable for the analysis of large amount of grass pea samples.关键词
山黧豆/还原型谷胱甘肽/邻苯二甲醛/荧光分光光度法Key words
Lathyrus sativus L / red uced glutathione/ o- phthalaldehyde/ fluorospectrophotometry.分类
轻工纺织引用本文复制引用
杨玲娟,董晓宁,王小芳,焦成瑾,顾水英..山黧豆中还原型谷胱甘肽的荧光检测[J].食品工业科技,2011,(4):397-399,404,4.基金项目
国家自然科学基金资助项目(30625025,30270965) （30625025,30270965）
甘肃省教育厅基金项目(1008B-07). （1008B-07）
