中国食品添加剂Issue(1)：110-115,6.
海藻酸钠固定化β-葡萄糖醛酸苷酶的研究
Study on the immobilization of β-glucuronidase by sodium alginate
摘要
Abstract
β - glucuronidase was immobilized by sodium alginate as the carrier. The method of cross - linking - embedding was adopted to immobilize β - glucuronidase. The effects of reaction conditions ( the concentration of sodium alginate, glutaraldehyde concentration, CaCl2concentration, cross - linking time and immobilized time) on the relative activity of immobilized β - glucuronidase were tested. The orthogorad test was used to investigate the optimal condition of immobilization. The results showed that the best efficiency of immobilization was obtained when sodium alginate concentration was 35 g/L, β - glucuronidase dosage was 1600 U, glutaraldehyde concentration was 0. 2％, CaCl2 concentration was 20g/L, and immobilized time was 2h. The recovery rate of immobilized enzyme activity was 71.66 ％.The study showed that this immobilize method was simple and had a prospective industrial application.关键词
β-葡萄糖醛酸苷酶/海藻酸钠/固定化酶分类
轻工纺织引用本文复制引用
张斌,孙倩,任延鹏,梁艳明,张婉婧,陈璐,李春,曹红..海藻酸钠固定化β-葡萄糖醛酸苷酶的研究[J].中国食品添加剂,2011,(1):110-115,6.基金项目
2009年度国家大学生创新性实验计划项目"离子液体中酶法合成GAMG的研究"、 教育部科学技术研究重点项目"固定化β-葡萄糖醛酸苷酶在离子液体中催化合成GAMG的研究"(209146) （209146）
2008年度石河子大学高层次人才科研启动资金专项"酶膜生物反应器制备GAMG的研究"(RCZX200805)资助. （RCZX200805）
