中国食品添加剂Issue(1)：156-159,4.
番石榴叶中多糖分离纯化工艺研究
Study on the optimal extraction and purification technology for polysacchrides from the guava leaves
杜阳吉 1王三永 1李春荣1
作者信息
- 1. 广东省食品工业研究所,广东省食品工业公共实验室,广州,510308
- 折叠
摘要
Abstract
In order to find out the best method of isolation and purification of polysaccharide from the guava leaves, the best extraction method was determined by orthogonal design. The result showed that the best extraction and purification method for the polysaccharide was determined as follows: At first, the guava leaves was soaked in water for 3 hours at 80 ℃ and extracted for three times. The ratio of the solvent (water) and the material ( guava leaves) was 30: 1 (w/w). The crude polysacchride was further purified by the extraction of ethanol and followed by the absorbtion. We also studied the ability of purifying polysaccaride from the guava leaves by macroporous absorption resin. First, the crude polysaccaride was purified by macroporous absorption resin (Diaion HP -20) using the ethanol/H2O system;then, the polysaccaride was purified by ethanol/H2O system and dried. The amount of the polysaccaride was determined by phenol - sulphuric acid method. The results showed that the purification was higher by the absorbtion of macroporous absorption resin ( Diaion HP - 20) and the extraction of ethanol. The macroporous absorption resin ( Diaion HP- 20) showed a good ability of absorption of polysaccaride (50g/L). It can effectively absorb the polysaccaride. Furthermore, the method showed a good ability of recovery of polysaccharide. Under this condition, the content of polysaccaride in the final product was 95.64％.关键词
番石榴叶/多糖/提取/纯化/含量测定分类
轻工纺织引用本文复制引用
杜阳吉,王三永,李春荣..番石榴叶中多糖分离纯化工艺研究[J].中国食品添加剂,2011,(1):156-159,4.
