中国食品学报2011，Vol.11Issue(1)：7-13,7.
茶叶糖蛋白调控树突状细胞对T淋巴细胞增殖和CTL活性的影响
Regulatory Effect of Tea Glycoprotein on Proliferation of T lymphocyte and Activity of CTL Induced by Dendritic Cells
摘要
Abstract
To observe the effects of tea glycoprotein(TGP) on the surface molecules expression level and function of murine bone marrow derived dendritic cells. DCs generated from Balb/e murine bone marrow cells were induced by rmGM-CSF and rmIL-4. TGP was added to cells on day 6 of culture for 48 h. Morphology development of DCs was observed by inverted microscope. The phenotypes of DCs were analyzed by using flow cytometry. Antigen presenting ability to allogeneic na(i)ve or syngeneie primed T lymphocytes was examined by the lymphocyte proliferation of mixed lymphocyte reaction (MLR) by using MTT assay. Investigate the effect of TGP on cytotoxicity of specific cytotoxic Tlymphocytes (CTL) of SP2/0 tumor cell induced by DCs. The results showed that TGP treated DCs displayed a more matured morpha, with long protrusions, while untreated-DCs displayed shorter protrusions than stimulated DCs. TGP treated immature DCs showed an enhanced cell-surface expression of MHC Ⅱ on CD11c gated DCs. The result demonstrated that TGP can increase antigen presenting ability of DCs to allogeneically na(i)ve or syngeneically primed T lymphoeytes. It was a conclusion that TGP can promote the cytotoxicity of specific CTL induced by DCs which pulsed with SP2/0 tumor antigen durning the stage of antigen presentation. The results showed that TGP promoted LDH activities released into eulturo supernatants. TGP can induce phenotypic and functional maturation of murine dendritic cells.关键词
茶叶糖蛋白/树突状细胞/表型/混合淋巴反应/抗原提呈/细胞毒性丁淋巴细胞引用本文复制引用
韩澄,聂少平,黄丹菲,陈一晴,谢明勇..茶叶糖蛋白调控树突状细胞对T淋巴细胞增殖和CTL活性的影响[J].中国食品学报,2011,11(1):7-13,7.基金项目
教育部长江学者和创新团队发展计划(No.IRT0540) （No.IRT0540）
食品科学与技术国家重点实验室目标导向项目(SKLF-MB-200806) （SKLF-MB-200806）
食品科学与技术国家重点实验室自由探索项目(SKLF-TS-200813)资助 （SKLF-TS-200813）
