中国食品学报2011，Vol.11Issue(1)：28-33,6.
溶剂与提取方式对苦荞提取液抗氧化性能的影响
Effects of Extraction Solvents and Methods on Antioxidant Activity of Tartary Buckwheat Bran Extracts
摘要
Abstract
Objective: In order to search a efficient method for extracting antioxidant from tartary buckwheat bran,which applies to laboratory. Methods: The effects of different solvent systems (80％ methanol, 60％methanol, 80％ethanol, absolute ethanol, 80％ acetone, 50％ acetone) and extraction procedures (homogenization extraction, heat reflux extraction, ultrasound extraction, maceration extraction and agitation extraction) on total phenol and total flavonoids contents and antioxidant activities in bran extracts of tartary buckwheat were investigated by Folin-ciocalteu's methods and three different bioassays: 2,2'-azino-bis(3-ethylbenzothiazo-line-6-sulfonic acid)diammonium salt(ABTS), 2,2'-diphenyl-1-picrylhydrazyl(DPPH) radical scavenging assays and β-carotene-linoleic acid antioxidant system, respectively. Result:80％ acetone extracts exhibited highest total flavonoids contents (3 361.28 mg Rutin eq/100 g DW) and the strongest antioxidant activities in DPPH· assays (24.53 mmol Trolox eq/l00g DW). Antioxidant activities of ultrasound extracts was found to be higher than others measured in the terms of trolox equivalent antioxidant capacity(30.34 mmol Trolox eq/l00g DW, 30.47 mmol Trolox eq/l00 g DW) with ABTS·+, DPPH· assays and in terms of inhibition using β-carotene-linoleic acid assay (antioxidant activity coefficient, AAC=912.15). Conclusion: 80％ acetone and ultrasound extract, which were a better choice for extracting antioxidants from buckwheat bran, showed relatively high phenolic contents and strong antioxidant activities.关键词
苦荞/提取溶剂/提取方式/总酚/抗氧化能力引用本文复制引用
杨红叶,柴岩,黄忠民,王敏..溶剂与提取方式对苦荞提取液抗氧化性能的影响[J].中国食品学报,2011,11(1):28-33,6.基金项目
"十一五"国家科技支撑计划项目(No.2006BAD02B06) （No.2006BAD02B06）
