食品与发酵工业2011，Vol.37Issue(2)：62-64,69,4.
条斑紫菜蛋白酶解物降血压活性
Study on Antihypertensive Activity of Bioactive Oligopeptide from Porphyra yezoensis Proteins
摘要
Abstract
In the paper, the preparation of bioactive oligopeptides from Porphyra yezoensis proteins in the presence of papain was studied.The ACE inhibitory activity was used as response to optimize the enzymatic conditions.The molecular weight distribution of the hydrolysates was determined by gel filtration chromatography on SephadexG-15 column.The result of which showed that the optimum conditions were pH 7.5, temperature 50℃, substrate concentration 30mg/mL, addition amount of enzyme 600 U/g.Under such conditions, and hydrolyzing for 4 hours, the inhibitory activity of the hydrolysates reached up to 30％, with IC50 value reaching 4.48mg/mL.The hydrolysates of Porphyra yezoensis were then determined by chromatography, the result of which showed that the hydrolysates of antihypertensive activity from Porphyra yezoensis proteins was mainly oligopeptide with molecular weight less than 2000.关键词
条斑紫菜/ACE抑制活性/木瓜蛋白酶引用本文复制引用
姚兴存,蒋栋磊,盘赛昆,舒留泉..条斑紫菜蛋白酶解物降血压活性[J].食品与发酵工业,2011,37(2):62-64,69,4.基金项目
江苏省海洋生物技术重点建设实验室开放课题(2008HS015) （2008HS015）
