食品与发酵工业2011，Vol.37Issue(2)：160-164,5.
应用流式细胞技术快速定量检测UHT奶产品中的微生物
Quantitatively Detect Microorganism in UHT Milk Rapidly by Using Flow Cytometry
摘要
Abstract
In this research we distinguish the live cells, dead cells and other large particles accurately in the UHT milk samples using a new Fluorescence Labeling Technique basing on single live cell, and quickly detect microorganism quantitatively by using flow cytometry.Comparison with traditional plate counting method shows that the detection range of FCM is 101 ～ 107 CFU/mL, far above that of plate counting method.Correlation analysis of the results of the two methods shows that within certain concentration range of bacteria solution, the results of FCM and plate counting method are linear, and FCM is a more accurate and rapid detection method for different types of microorganism.关键词
流式细胞术/UHT奶/微生物/定量检测引用本文复制引用
孙晓霞,赵占民,刘道亮,张峻峰..应用流式细胞技术快速定量检测UHT奶产品中的微生物[J].食品与发酵工业,2011,37(2):160-164,5.基金项目
国家质检总局科技计划项目(No.2009IK174) （No.2009IK174）
