食品与发酵工业2011，Vol.37Issue(2)：182-185,4.
柱前衍生超高效液相色谱法测定核桃仁中的氨基酸含量
Determination of Amino Acids in Walnuts by Ultra Pefformanace Liquid Chromogray(UPLC)with Pre-column Derivation
摘要
Abstract
To establish an UPLC method in determination the amounts of Amino Acids in Walnuts.A Waters Acquity UPLC BEH C18 column (2.1 mm × 150 mm, 1.7μm) was applied,using AccQ.Tag Ultraa eluent A and AccQ.Tag Ultraa eluent B as the mobile phase,with a flow rate of 0.7 mL/min,and the column temperature 55C.The calibration curve was linear in the range of 0.003 ～ 0.18 mg/mL of Amino Acids (r ＞ 0.999 4).The average recovery of Amino Acids was 98.5％.It has the advantage of simple, accuracy, and repeatable in the determination Amino Acids.关键词
超高效液相色谱(UPLC)/氨基酸/核桃仁/含量测定引用本文复制引用
杨俊,王文辉,李翼,王齐,孙晓东,朱艳琴..柱前衍生超高效液相色谱法测定核桃仁中的氨基酸含量[J].食品与发酵工业,2011,37(2):182-185,4.基金项目
昆明理工大学分析测试研究中心主任基金(2008-002) （2008-002）
