食品与发酵工业2011，Vol.37Issue(2)：7-10,4.
酸性普鲁兰酶基因在地衣芽孢杆菌中的表达
Expression of the Acid Pullulanase in Bacillus licheniformis
摘要
Abstract
According to the sequence of pullulanase gene from Bacillus deramificans ( NCBI accession number:AX203843 ), the gene encoding mature peptide of pulluanase was synthesized and designated pulA. The puIA was amplified by the method of PCR and cloned into the expression vector pHY - WZX, yielding hybrid plasmid pHY-WZX- pulA. Subsequently, pHY- WZX -pulA was introduced into Bacillus licheniformis B60608. Active pullulanase was expressed by recombiant B. licheniformis and secreted into medium. Culture condition of recombinant B. licheniformis were optimized for production of pullulanase. The optimized medium consists of 2 ％ cotton seed protein and 8 ％ of glycerol.关键词
普鲁兰酶/地衣芽孢杆菌/Bacillus deramificans引用本文复制引用
谢银珠,沈微,王正祥..酸性普鲁兰酶基因在地衣芽孢杆菌中的表达[J].食品与发酵工业,2011,37(2):7-10,4.基金项目
国家863高技术研究发展计划(2006AA10Z307) （2006AA10Z307）
