食品与发酵工业2011，Vol.37Issue(2)：21-23,3.
一种酵母快速批量分子鉴定方法
A Rapid Method for Yeast Molecular Identification
摘要
Abstract
D1/D2 domain of 26S rDNA sequence-based alignment and analysis is a widely used method for yeast identification and classification.Rapid and repeatable protocols to extract and prepare the chromosomal DNA of yeast are the basic needs to acquire the templates for following amplification of D1/D2 domain.However, the present methods to extract chromosomal DNA are time -consuming and unable to deal with large-scale samples at the same time.In the present study, a novel rapid and efficient method to identify a large amount of yeast isolates in the same batch was established.The chromosomal DNA required only 20 minutes to prepare and can be directly used as template for PCR amplification.Results of amplified ITS sequences were clear and easy to identify by sequencing analysis.关键词
酵母/染色体DNA/提取/26S rDNA D1/D2区域/鉴定引用本文复制引用
陈源源,石贵阳,王正祥..一种酵母快速批量分子鉴定方法[J].食品与发酵工业,2011,37(2):21-23,3.基金项目
国家自然资源科技平台(No.2005DKA21208)资助 （No.2005DKA21208）
