中国食品学报2011，Vol.11Issue(2)：147-151,5.
介孔分子筛SBA-15固定糖化酶的研究
Immobilization of Glucoamylase on Mesopore Molecular Sieve SBA-15
摘要
Abstract
The immobilization of glucoamylase was carried out using glutaraldehyde as crosslink agent, using SBA-15 as carrier. The effect of immobilization temperature and time enzyme addition, pH value and glutaraldehyde concentration on immobilization result were investigated. The optimal immobilization conditions were determined. The results were as follows: the ratio of enzyme to carrier was 50 mg to 1.0 g, immobilization temperature 25 ℃, immobilization time 4h,pH 5.1, and the concentration of glutaraldehyde7.5％. Under these optimal conditions, activity recovery of immobilized enzyme was 56％. And the enzymatic properties of the immobilized enzyme were studied.The results showed that the optimal temperature of immobilized enzyme was 70℃, pH4.1, Km 0.022mol/L.The activity of immobilized enzyme was increased 18％ after treated by ultrasonic. The best ultrasonic parameters obtained were as follows: ultrasonic power 100 W, temperature 70 ℃, and time 5 min.关键词
介孔分子筛/糖化酶/固定化/超声波Key words
mesopore molecular sieve/ glucoamylase/ immobilization/ ultrasonic引用本文复制引用
侯红萍,张茜..介孔分子筛SBA-15固定糖化酶的研究[J].中国食品学报,2011,11(2):147-151,5.基金项目
山西省科技厅攻关项目(041030) （041030）
