食品与发酵工业2011，Vol.37Issue(3)：94-98,5.
毕赤酵母工程菌pk53产纤溶酶发酵条件的优化
Study on the Fermentation for Fibrinolytic Enzyme from Pichia pastoris pk53
摘要
Abstract
The fermentation medium and conditions of the strain were studied via single factor test and orthogonal design.The optimal fermentation medium was determined as follows: glucose 1.5％, soypeptone 2.0％, KH2PO4 0.5 ％ , MgSO4 · 7H2 O 0.05 ％.The best fermentation conditions were:inoculating time 14 h, inoculating amount 1％ ,methanol supply 1.5％ , medium volume 30 mL in 250 mL flask,initial pH 5.5.Under such conditions,the activity of fibrinolytic enzyme (corresponding to the unit of urokinase activity) was 429.06 U/mL,which was 8.88 times of the initial strain.关键词
毕赤酵母/纤溶酶/发酵/优化Key words
Pichia pastoris /fibrinolytic enzyme/ fermentation/ optimization引用本文复制引用
焦龙,于宏伟,郭润芳,白玉,贾英民..毕赤酵母工程菌pk53产纤溶酶发酵条件的优化[J].食品与发酵工业,2011,37(3):94-98,5.基金项目
河北省十一五农产品加工富民工程专项课题:农产品加工用高效微生物菌种培育及菌种资源库建设(06220106D) （06220106D）
