沈阳农业大学学报2011，Vol.42Issue(1)：31-36,6.
玉米顶腐镰孢菌rDNA-ITS和EF-1a基因序列及UP-PCR遗传多样性分析
rDNA-ITS and EF-1a Gene Sequences of Fusarium species Causing Maize Top Rot and UP-PCR Analysis on Genetic Diversity
摘要
Abstract
A total of 89 Fusarium isolates were obtained from 45 diseased plants of maize top rot collected from some regions of Liaoning Province. Using traditional morphological classification and rDNA-ITS and EF-1a gene sequence analysis methods, 4 Fusarium species, Fusarium. subglutinans, F. verticillioides, F. proliferatum and F. oxysporum, were identified. Twenty-three isolates were clustered into 4 groups based on EF-1a gene sequence on phylogenetic tree. Additionally, genetic diversity of Fusarium species was analyzed using universally primed PCR (UP-PCR). The result indicated that a total of 57 bands appeared after amplification by using 5 primers, 53 bands (93.0％) of which were polymorphic. Twenty-four isolates were clustered into 4 groups at the similarity of 0.626 by UP-PCR cluster analysis. Our results indicated that EF-1a gene sequence could reflect the genetic diversity and differences of Fusarium species.关键词
玉米顶腐镰孢菌/ITS/EF-1α基因/通用引物PCR/遗传多样性Key words
Fusarium species/ ITS/ EF-1a gene/ universally peimed PCR/ genetic diversity分类
农业科技引用本文复制引用
陈璐,高增贵,庄敬华,张硕,高金欣..玉米顶腐镰孢菌rDNA-ITS和EF-1a基因序列及UP-PCR遗传多样性分析[J].沈阳农业大学学报,2011,42(1):31-36,6.基金项目
国家科技支撑计划项目(2006BAD08A06) （2006BAD08A06）
国家粮丰工程项目(2011BAD16B12) （2011BAD16B12）
辽宁省教育厅重点实验室项目(LS2010149) （LS2010149）
