食品与机械2011，Vol.27Issue(3)：52-55,67,5.DOI:10.3969/j.issn.1003-5788.2011.03.016
大孔吸附树脂纯化金银花叶总多酚的工艺优化
Process optimization on purification of total polyphenols in loniceram acranthoides with macroporous adsorption resins
摘要
Abstract
To evaluate the absorption capability and purification effect of polyphenols on macroporous absorption resin,and to find a better method to separate and purify total polyphenols in Loniceram acranthoides leaves.Using Folin-Ciocalteu as polyphenols determination method, the more suitable resin for purification of total polyphenols in Loniceram acranthoides leaves was selected by comparing 8 kind of macroporous adsorption resin’s adsorption and desorption capacity and optimum conditions for absorption and elution of total polyphenols were performed..The results showed that NKA-Ⅱ is the more suitable medium to purify total polyphenols, the optimum condition to purify total polyphenols with NKA- Ⅱ was to control the flow velocity at 2 BV/h, pH value at 3, concentration of feed at 6 mg/mL and with 60％ ethanol as washing solution,the total polyphenols.produced by this process was pink powder with total polyphenols.purity up to 61.88％.关键词
金银花叶/总多酚/大孔吸附树脂/纯化Key words
leaf of lonicera japonica / total polyphenols/ macroporous absorption resin/ purification引用本文复制引用
熊建华,汤凯洁,罗秋水,董开发..大孔吸附树脂纯化金银花叶总多酚的工艺优化 [J].食品与机械,2011,27(3):52-55,67,5.基金项目
国家自然科学基金项目(编号:31060226) （编号:31060226）
江西省科技支撑项目(编号:2009BGB00900) （编号:2009BGB00900）
江西农业大学自然科学基金项目(编号:2009-2944) （编号:2009-2944）
