食品与机械2011，Vol.27Issue(3)：76-78,87,4.DOI:10.3969/j.issn.1003-5788.2011.03.023
RP-HPLC法测定核桃外果皮中齐墩果酸的含量
Determination of oleanolic acid in walnut pericarp by RP-HPLC
摘要
Abstract
The method was established for the determination of oleanolic acid in walnut pericarp by RP-HPLC, with the chromatographic column of intersil ODS( 150 mm × 4.6 m, 5μm), the mobile phase consisted of methanol∶ water∶ acetic acid (265∶ 35 ∶ 0.1 ,V/V) at the flow rate of 0.8 mL/min; the injection volume was 20 μL; the detection wavelength was 210 nm and the column temperature was 30 ℃.The results showed that the good linear range of oleanolic acid was 0.728～7.28 μg (r = 0.999 7), and the average recovery (n =9) rate was 101.1％ with RSD of 2.44％.The content of oleanolic acid in walnut pericarp was 2.1 mg/100 g.This method is simple and quick.The result is accurate and repeatable.关键词
反相高效液相色谱法/核桃外果皮/齐墩果酸Key words
RP- HPLC/ walnut pericarp / oleanolic acid引用本文复制引用
张瑞廷,徐佳,傅力..RP-HPLC法测定核桃外果皮中齐墩果酸的含量 [J].食品与机械,2011,27(3):76-78,87,4.基金项目
国家"十一五"科技支撑计划项目(编号:2007BAD36B04) （编号:2007BAD36B04）
新疆维吾尔自治区高校科研计划科学研究重点项目(编号:XJEDU2008I13) （编号:XJEDU2008I13）
