现代食品科技2011，Vol.27Issue(6)：719-722,4.
应用LAMP快速检测金黄色葡萄球菌的研究
Loop-mediated Isothermal Amplification Assay for Rapid Detection of Staphylococcus Aureus
摘要
Abstract
The presence of Staphylococcus aureus in food represents a potential risk to public health, being its exterotoxins the major virulence factor. The purpose of this study was to develop a loop-mediated isothermal amplification (LAMP) method for the rapid, sensitive and simple detection of Staphylococcus aureus in food. LAMP primers were designed by targeting the nuc gene. With Bst DNA polymerase, the target DNA can be clearly amplified for 45 min at 65 degree C. LAMP products could be judged with agar gel or naked eye after the addition of SYBR Green I. The assay correctly identified Staphylococcus aureus, but did not detect 8 non- Staphylococcus aureus strains. In this way, 240 egg samples were investigated for Staphylococcus aureus. The accuracy of LAMP was shown to be 100％ when compared to the standard method. Consequently, the LAMP assay is a sensitive, rapid and simple tool for the detection of Staphylococcus aureus and will facilitate the surveillance for control of contamination of Staphylococcus aureus in food.关键词
金黄色葡萄球菌/环介导等温扩增技术/nuc基因Key words
Staphylococcus aureus/ Loop-Mediated Isothermal/ Amplification/ nuc gene引用本文复制引用
唐梦君,周生,葛庆联,张小燕,蒲俊华,高玉时..应用LAMP快速检测金黄色葡萄球菌的研究[J].现代食品科技,2011,27(6):719-722,4.基金项目
江苏省科技创新与成果转化专项引导资金(No.BM2009908) （No.BM2009908）
国家蛋鸡产业体系(NYCYTX-41-28) （NYCYTX-41-28）
江苏省家禽科学研究所博士基金(JQ201002) （JQ201002）
