中国食品学报2011，Vol.11Issue(3)：192-196,5.
生物转化体系中天麻素和对羟基苯甲醇同步检测方法研究
Simultaneous Determination of Gastrodin and p-2-Hydroxybenzyl Alcohol in Biotransformation System
摘要
Abstract
Objective: To establish a method for simultaneous determination of Gastrodin and p-2-hydroxybenzyl methanol in biotransformation system. Method: RP-HPLC was used in the study, the mobile phase consists of 0.02％phosphoric acid solution (pH 2.6) and methanol (5:95, V/V), the flow rate was 0.5 mL/min, all chromatographic operations were carried out at the temperature of 35 ℃ with UV detector at 220 nm. Results: Under the above condition, good linearity was obtained for both Gastrodin and p-2-hydroxybenzyl alcohol with a correlation coefficient 0.9999, recoveries ranged between 95％ and 102％, while the precision of the method was ＜5％ (RSD). Conclusion: The content of gastrodin and p-2-hydroxybenzyl alcohol in biotransformation system can be accurately determine simultaneously by this method.关键词
天麻素/对羟基苯甲醇/同步检测/反相高效液相色谱Key words
gastrodin/ p-2-hydroxybenzyl alcohol/ simultaneous determination/ RP-HPLC引用本文复制引用
傅明亮,章海锋,刘晓杰,刘婧,陈启和..生物转化体系中天麻素和对羟基苯甲醇同步检测方法研究[J].中国食品学报,2011,11(3):192-196,5.基金项目
国家自然科学基金项目(No.20806069) （No.20806069）
浙江省科技计划面上项目资助(No.2009C32098) （No.2009C32098）
