食品与发酵工业2009,Vol.35Issue(9):88-92,5.
玉米粗肽的可控酶法制备及其体外醒酒活性的研究
Controlled-enzymatic Preparation of Crude Corn Peptide and Its Antialcoholism Activity in vitro
摘要
Abstract
Crude corn peptides were prepared via enzymatic hydrolysis of corn proteins by different proteases and their effects on the ADH activation ratios were compared. Among the examined proteases,Alcalase showed as the best catalyst,with which the enzymatic hydrolysis conditions were optimized. Results showed that the optimal enzyme dosage,substrate concentration reaction conditions and reaction time were 1%,5%,60℃ and 120min,respectively,under which the DH and peptide yield of the reaction reached 48. 4% and 58. 6%,respectively. Study of its antialco-holism activity in vitro showed that the highest ADH activation ratio was 38. 5% when the DH and peptide yield reached 30.0% and 57.0%,respectively.关键词
玉米蛋白粉/玉米租肽/酶解/Alcalase/ADH激活率Key words
corn proteins/corn peptide/zymohydrolysis/Alcalase/ADH activation ratio分类
轻工纺织引用本文复制引用
吴晖,王蓓蓓,李丽丽,李晓凤,余以刚,刘冬梅,肖性龙..玉米粗肽的可控酶法制备及其体外醒酒活性的研究[J].食品与发酵工业,2009,35(9):88-92,5.基金项目
教育部新世纪优秀人才支持计划项目(NCET-060746),教育部高校博士点专项科研基金(200805611022). (NCET-060746)