食品与发酵工业2009，Vol.35Issue(9)：134-136,141,4.
阳离子交换树脂分离纯化酶法合成的双果糖酐Ⅲ
Separation and Purification of Difructose Anhydride Ⅲ Prepared by Enzymatic Synthesis Using Cation Exchange Resin
摘要
Abstract
ABSTRACT The separation and purification of difructose anhydride Ⅲ prepared by enzymatic synthesis using cation exchange resin DTF-02 was studied. The influence of column temperature,sample concentration,sample volume and elution flow rate were discussed. The study showed that,when 5mL difructose anhydride Ⅲ containing a soluble solid content of 20%,difructose anhydride Ⅲ was completely separated from fructo-oligosaccharide with elution flow rate of 1 mL/min at 60 ℃. Under such condition,the fractions containing DFA Ⅲ were separated and determined by HPLC which showed DFA Ⅲ content was over 95%. The research laid theoretical foundations for large-scale separation and purification of difructose anhydride Ⅲ.关键词
双果糖酐Ⅲ/低聚果糖/阳离子交换树脂/分离纯化Key words
difructose anhydride Ⅲ/fructo-oligosaccharide/cation exchange resin/separation/purification分类
轻工纺织引用本文复制引用
赵萌,张涛,沐万孟,江波,卢峥嵘,杜倩..阳离子交换树脂分离纯化酶法合成的双果糖酐Ⅲ[J].食品与发酵工业,2009,35(9):134-136,141,4.基金项目
"十一五"国家863资助项目(2006AA10Z334) （2006AA10Z334）
