高效液相法同时测定混合环糊精中的α,β,γ-环糊精的含量OA北大核心CSCDCSTPCD
HPLC For Simultaneously Determination of a Mixed-cyclodextrin of α,β,γ-Cyclodextrin
建立了同时测定环糊精中α-环糊精、β-环糊精、γ-环糊精3种成分含量的高效液相色谱法.方法采用色谱柱为SpherrigeL C_6H_5(5μm,300 mm×3.9 mm),流动相V(甲醇):V(水)=5:95进行洗脱,流速为1.0 mL/min,检测器为示差折光检测器,柱温40℃,进样量20μL.α-环糊精、β-环糊精、γ-环糊精的检出限分别为1.03,2.53,1.50μg/g,线性范围分别为2.0-207.9、11.5-230、3.9-1…查看全部>>
An HPLC method for determination of three kinds of cyclodextrins, a-cyclodextrin, p-cyclodextrin, γ-cyclodextrin was developed. The system was ; Spherrigel C_6H_3 column (5 u,m, 300 mm×3. 9 mm) , methanol-water (5:95, v/v)as the solvent, the flow rate of 1.0 mL/min(column temperature 40℃)with the refractive index detector. The results were obtained that the detection limit of a-CD, β-CD, γ-CD were 1. 03 μg/g,2. 53 μg/g,1.50 u,g/g, linear range of the three k…查看全部>>
毛勇;邓媛;李皎;汪大敏;杨国武
陕西省微生物研究所,陕西,西安,710043陕西省微生物研究所,陕西,西安,710043陕西省微生物研究所,陕西,西安,710043陕西省微生物研究所,陕西,西安,710043陕西省微生物研究所,陕西,西安,710043
轻工纺织
高效液相色谱法α-环糊精β-环糊精γ-环糊精含量测定
HPLC a-cyclodextrin β-cyclodextrinγ-cyclodextrin determination
《食品与发酵工业》 2009 (10)
138-140,3
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