食品与发酵工业2009，Vol.35Issue(11)：56-59,4.
产γ-氨基丁酸菌株的分离和选育
Screening and Breeding of γ-Aminobutyric Acid-Producing Microorganisms
冯宇 1张颖 1潘超强 1任雪 1赵文怡 1高年发1
作者信息
- 1. 工业微生物教育部重点实验室,天津科技大学生物工程学院,天津,300457
- 折叠
摘要
Abstract
Strain F is obtained, yielding 16 g/L γ-aminobutyric acid (GABA), from China traditional fermented vegetables. According to its colony morphology, physiological and biochemical properties and homology of 16S rDNA,strain F is identified as Lactobacillus brevis, and named as Lactobacillus brevis TCCC13007. The 30% positive mutation rate occurs when it is stimulated by diethyl sulfate and lithium chloride simultaneously for 17mins, and the mortality is more than 95%. After that, strains which grow in GYP agar medium with 50 g/L GABA inside are selected randomly, so that a 24 g/L GABA-yielding strain F1 is obtained by determining the content of GABA in fermentation culture. Its productivity is 50% more than the original and confirmed to be stable after an 8 times successive fermentation test.关键词
γ-氨基丁酸/短乳杆菌/鉴定/诱变Key words
γ-aminobutyric acid/ Lactobacillus brevis/ identification/ mutagenesis分类
轻工纺织引用本文复制引用
冯宇,张颖,潘超强,任雪,赵文怡,高年发..产γ-氨基丁酸菌株的分离和选育[J].食品与发酵工业,2009,35(11):56-59,4.
