食品科学2011，Vol.32Issue(13)：173-178,6.
复合酶-微波辅助萃取结合超滤纯化的灰树花子实体多糖的免疫活性研究
Immunoactivity of Grifola frondosa Fruit Body Polysaccharides Prepared by Microwave-assisted Extraction followed by Multi-enzyme Hydrolysis and Purified by Ultrafiltration
摘要
Abstract
Objective：To explore the immunoactivity of Grifola frondosa fruit body polysaccharides（GFP） with different relative molecular weights.Methods：Three polysaccharide fractions from Grifola frondosa fruit body named as GFP-I（ 50 kD）,GFP-II（50-100 kD） and GFP-III（100-200 kD） were prepared by microwave-assisted extraction followed by multi-enzyme hydrolysis and purified by ultrafiltration.The proliferation of murine lymphocytes with and without Con A or LPS induction in virto was evaluated by MTT assay.A S180 tumor-bearing mouse model was established,and the inhibitory effect of the three GFP fractions at different concentrations on tumor growth was also explored.Results：Lymphocyte proliferation rate without induction was increased by 26.558%-74.167%（P0.001）,20.175%-152.075%（P0.001） and 26.473%-334.797%（P0.001）,respectively in group GFP-I,GFP-II and GFP-III as compared with RPMI-1640 group,Con A-induced lymphocyte proliferation rate exhibited respective increases of 31.408%-122.894%（P0.001）,53.075%-235.693%（P0.001） and 83.347%-367.241%（P0.001） for group GFP-I,GFP-II and GFP-III,and LPS-induced lymphocyte proliferation rate was increased by 20.526%-97.075%（P0.001）,38.288%-211.348%（P0.001） and 54.454%-344.883%（P0.001）,respectively.In comparison with NS group,the inhibitory rates of GFP-I,GFP-II and GFP-III on tumor growth in S180 tumor-bearing mice were 10.15%-22.94%（P0.001）,12.79%-30.10%（P0.001） and 23.86%-51.65%（P0.001）,respectively.Conclusion：GFP-I,GFP-II and GFP-III have notable immunoactivity and anti-tumor activity and the activity of GFP-III is the strongest and positively related to its concentration.Further,these three polysaccharides might mainly promote the T lymphocyte proliferation.关键词
灰树花子实体多糖/复合酶-微波辅助萃取/超滤/免疫活性/肿瘤抑制率Key words
Grifola frondosa polysaccharides/microwave-assisted extraction followed by multi-enzyme hydrolysis/ultrafiltration/immunoactivity/inhibitory rate of tumor growth分类
医药卫生引用本文复制引用
刘红梅,李栋,樊梦丹..复合酶-微波辅助萃取结合超滤纯化的灰树花子实体多糖的免疫活性研究[J].食品科学,2011,32(13):173-178,6.基金项目
北京市属高等学校人才强教计划资助项目 （）
