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南极磷虾自溶酶性质的初步研究

杭虞杰 李学英 杨宪时 郭全友

食品科学2011,Vol.32Issue(13):198-200,3.
食品科学2011,Vol.32Issue(13):198-200,3.

南极磷虾自溶酶性质的初步研究

Properties of Autolysis Enzymes from Antarctic Krill

杭虞杰 1李学英 2杨宪时 2郭全友2

作者信息

  • 1. 上海理工大学医疗器械与食品学院,上海200093
  • 2. 中国水产科学研究院东海水产研究所,上海200090
  • 折叠

摘要

Abstract

The optimal pH and temperature of autolysis enzymes from Antarctic krill were investigated in this paper.Meanwhile,the effects of inhibitors and metal ions on the activities of autolysis enzymes from Antarctic krill were also explored.The results showed that autolysis enzymes from Antarctic krill were mainly composed of three enzymes with respective optimal reaction pH of 3.0,6.0 and 8.0.In addition,the optimal reaction temperatures of the three enzymes were determined to be 40,50 ℃ and 50-60 ℃,respectively.phenylmethyl sulfonyl fluoride(PMSF) and soybean trypsin inhibitor(SBTI) exhibited an obvious inhibition effect on autolysis enzymes from Antarctic krill at a pH ranging from 3.0 to 9.0 and from 6.0 to 10.5,respectively.Moreover,Cu2+ and Ba2+ could strongly inhibit the activities of the autolysis enzymes although Ca2+ did not reveal inhibition effect.In contrast,the autolysis enzymes were activated by Mg2+.

关键词

南极磷虾/自溶酶/性质

Key words

Antarctic krill/autolysis enzyme/characterization

分类

生物科学

引用本文复制引用

杭虞杰,李学英,杨宪时,郭全友..南极磷虾自溶酶性质的初步研究[J].食品科学,2011,32(13):198-200,3.

基金项目

国家“863”计划项目 ()

中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院东海水产研究所)资助项目 ()

食品科学

OA北大核心CSCDCSTPCD

1002-6630

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