食品科学2011，Vol.32Issue(13)：201-204,4.
牛β-防御素BNBD11基因在大肠杆菌中的表达
Expression of Bovine Neutrophil β-Defensin BNBD11 Gene in Escherichia coli
摘要
Abstract
In order to explore the technical route for prokaryotic expression and purification of bovine neutrophilβ-defensin 11（BNBD11）,BNBD11 gene was synthesized using preferred condons of E.coli,according to the reported amino acid sequence of BNBD11.The recombinant expression plasmid pET32a-BNBD11 was constructed and then transformed into E.coli BL21.The expression of BNBD11 in E.coli BL21 was induced by isopropylβ-D-1-thiogalactopyranoside（IPTG）.SDS-PAGE showed that most expressed products were soluble.The fusion protein was purified by Ni Sepharose column chromatography.After the cleavage of the fusion protein by formic acid,Ni Sepharose column was used again to remove proteins with His-Tag and the purified recombinant BNBD11 was achieved.Therefore,BNBD11 was successfully expressed in E.coli,and the recombinant BNBD11 was obtained.关键词
β-防御素/BNBD11/融合表达/大肠杆菌Key words
β-defensin/BNBD1/fusion expression/Escherichia coli分类
生物科学引用本文复制引用
赵建乐,陈琛,闫茂仓,张小莺,李引乾,张三东..牛β-防御素BNBD11基因在大肠杆菌中的表达[J].食品科学,2011,32(13):201-204,4.基金项目
西北农林科技大学引进人才启动专项基金项目 （）
2009年西北农林科技大学基本科研业务费青年项目 （）
2010年温州市科技计划项目 （）
