食品科学2011，Vol.32Issue(14)：227-231,5.
新霉素ELISA检测方法的建立
Development of Enzyme-linked Immunosorbent Assay for Neomycin
摘要
Abstract
In this study, we describe the advantages and disadvantages of direct competitive enzyme-linked immunosorbent assay （dc-ELISA） and indirect competitive ELISA （idc-ELISA） and ELISA methods for the detection of neomycin. Antineomycin polyclonal antibodies were prepared and used to detect neomycin by dc-ELISA and idc-ELISA. The cross-reaction rates of prepared anti-neomycin polyclonal antibodies with gentamincin and kanamycin were 2.04% and 0.02%, respectively, and with ampicillin, erythromycin and tetracycline all less than 0.01%. The accuracy and recovery of idc-ELISA were tested with an intra-plate error of less than 4%, an inter-plate error of less than 11% and a recovery between 135.5% and 191.3%. The detection limits of dc-ELISA and idc-ELISA were 28.58 ng/mL and 51.74 ng/mL, respectively, both of which were below the national maximum residue limit （MRL） of 500 pg/kg. Therefore, a dc-ELISA method and an idc-ELISA method to detect neomycin have successfully established. Further, the idc-ELISA method where the working conditions were better optimized can be used for the development of neomycin test kit.关键词
新霉素/多克隆抗体/竞争酶联免疫法（enzyme/linked/immunosorbent/assay，ELISA）/方法建立Key words
neomycin/polyclonal antibodies/competitive enzyme-linked immunosorbent assay （ELISA）/method establishment分类
农业科技引用本文复制引用
刘沙洲,桑小雪,欧阳华学,雷绍荣,白林含..新霉素ELISA检测方法的建立[J].食品科学,2011,32(14):227-231,5.基金项目
四川省公益性研究计划项目 （）
