食品科学2011，Vol.32Issue(14)：303-306,4.
葛渣总黄酮提取及葛根素含量测定
Optimization of Total Flavonoids Extraction from Radix Puerariae and HPLC Determination of Puerarin
摘要
Abstract
In this paper, orthogonal array design L9（34） was employed to optimize the extraction conditions of total flavonoids from Radix Puerariae residue left after pueraria root powder extraction. The effects of extraction temperature, ethanol concentration, extraction time and material-to-liquid ratio on extraction rate of total flavonoids were probed. Meanwhile, a HPLC method to determine puerarin content in Radix Puerariae was presented based on optimized extraction process. The optimal extraction conditions were determined as follows： ethanol concentration 70%, reflux time 150 min, reflux temperature 80 ℃, and material-to-liquid ratio 1：40, under which, the contents of total flavonoids in Radix Puerariae, Radix Puerariae residue and pueraria root powder were 46.11, 32.41 mg/g and 11.23 mg/g, and of puerarin 40.63, 21.24 mg/g and 4.71 mg/g, respectively. From these results, we drew the conclusion that Radix Puerariae residue is rich in total flavonoids and puerarin and that the optimized extraction process can be used for the extraction of total flavonoids and puerarin from Radix Puerariae residue.关键词
葛渣/总黄酮/葛根素/高效液相色谱法/正交试验Key words
Radix Puerariae residue/total flavonoids/puerarin/high performance liquid chromatography： orthogohal array design分类
医药卫生引用本文复制引用
杨树平,韩立军,朱金卫,许瑞波,蒋迎道..葛渣总黄酮提取及葛根素含量测定[J].食品科学,2011,32(14):303-306,4.基金项目
江苏省海洋资源开发研究院科技开放基金课题 （）
淮海工学院自然科学基金项目 （）
淮海工学院引进人才启动基金项目 （）
