食品与发酵工业2011，Vol.37Issue(5)：35-39,5.
产壳聚糖酶海洋真菌鉴定、寡营养培养基优化及廉价培养基研究
Identification, Optimization of Oligotrophic Medium Fermentation Conditons of Chitosanase-producing Marine Strain, and Cheap Medium Research
摘要
Abstract
A chitosanase-producing marine strain was isolated by our laboratory. Identification by rDNA - ITS sequence analysis, and it is Penicillium oxalicum. This is the first time that Penicillium oxalicum＇ s the ability of producing chitosanase is reported. For the purpose of fermentation chitosanase in industrial, we studied oligotrophic medium components, such as source of carbon, nitrogen source, saltresistance, acidresistance and rotary speed. We explored different kinds of cheap medium for production of chitosanase. We found the soybean powder was the best, and the medium of soybean powder was as follow （g/L seawater） ： soybean powder 25 g, and the optimal enzyme activity was 8.5 U/mL. This medium was insufficient 5% of the cost of original. It provided direct theory and experiment foundation on industrialized production of chitosanase.关键词
Penicillium/oxalicum/壳聚糖酶/寡营养海水培养基/廉价培养基Key words
Penicillium oxalicum/chitosanase/oligotrophic seawater-medium/cheap medium分类
化学化工引用本文复制引用
朱利平,黄惠莉..产壳聚糖酶海洋真菌鉴定、寡营养培养基优化及廉价培养基研究[J].食品与发酵工业,2011,37(5):35-39,5.基金项目
福建省自然科学重点项目基金 （）
