食品与发酵工业2011，Vol.37Issue(5)：56-60,5.
豆油和丙氨酸对利福霉素SV生物合成的影响
Effect of Soybean Oil and Alanine Addition on Rifamycin SV Biosynthesis
摘要
Abstract
Effects of soybean oil and alanine on rifamycin SV biosynthesis in 250 mL flask were studied in this paper. When 3 mL/L soybean oil and 0.8 g/L alanine was added into the medium at 72 h, rifamycin SV production was increased by 5.3% and 6.9% over the control, respectively. Response surface methodology was used to study the interaction between soybean oil and alanine. Result showed that their optimal amount was 2.57 mL/L and 0. 89 g/ L, respectively. Under the optimal conditions, rifamycin SV production reached 5179 U/mL that was 12.3% higher than control. The results of organic acids analysis demonstrated that utilization rate of a-ketoglutarate and citriate was 0.86 mg/（L · h） and 0.77 mg/（L · h） , respectively, which was 115.7% and 23.3% higher than that of control in the fermentation process from 72 h to120 h. Meanwhile, succinate concentration reached 1.01 g/L that was 16.1% higher than the control. The result indicated that the conversion efficiency of α-ketoglutarate and citrate to succinate in the TCA cycle was greatly improved with the addition of oil and alanine, which led to enhanced synthesis of succinyl- CoA and methylmalony-CoA and the facilitation of rifamycin SV biosynthesis.关键词
豆油/丙氨酸/利福霉素SV/响应面分析法/有机酸Key words
soybean oil/alanine/rifamycin SV/response surface analysis/organic acid分类
化学化工引用本文复制引用
商纯良,王志,陈雄,王金华,黄亚男..豆油和丙氨酸对利福霉素SV生物合成的影响[J].食品与发酵工业,2011,37(5):56-60,5.基金项目
中国博士后科学基金（20080430865）项目 （）
