食品与发酵工业2011，Vol.37Issue(5)：73-78,6.
大环糊精制备条件的优化
Optimization of the Preparation of LR-CD
摘要
Abstract
Plackett-Burman design was used to select reaction conditions for large ring cyclodextrin （LR-CD） preparation, and the significant factors were optimized by Box-Behnken design. Results showed that, enzyme, temperature and time were three significant factors, t-test showed that model of Box-Behnken design was significant （P≤ 0.05 ）, and lack of fit was no significant （P≥0.05）. The regression coefficient R2 was 0.9404, which was showed a good regression result. The predicted yield of LR-CD was 56.58% , and the predicted conditions were enzyme 91.34 U/g, temperature 71.7℃ for 6.8 h. Validation test showed LR-CD yield of 55% , 2.3 folds higher than initial yield. Identification results showed that the degree of polymerization was ranged from 20 to 40, the ratio of cyclic component was 47 %.关键词
大环糊精/Plackett—Burman/因素筛选，Box—Behnken设计Key words
large ring cyclodextrin/plackett-burman/screen of factors/box-behnken design分类
轻工纺织引用本文复制引用
郭光辉,王金鹏,唐涛,徐学明,金征宇..大环糊精制备条件的优化[J].食品与发酵工业,2011,37(5):73-78,6.基金项目
国家自然科学基金 （）
江苏省自然科学基金 （）
江苏省自然科学基金创新学者攀登项目 （）
食品科学与技术国家重点实验窒2008年度目标导向项目 （）
