食品与发酵工业2011，Vol.37Issue(5)：160-165,6.
化学发光酶免疫法检测玉米中玉米赤霉烯酮
Chemiluminescence Enzyme Immunoassay for the Determination of Zearalenone in Corn
摘要
Abstract
A sensitive indirect competitive chemiluminescence enzyme immunoassay （CLEIA） for zearalenone in corn has been developed. The optimized CLEIA gave a limit detection of 0. 09 ng/mL and a detection range of 0. 104- 1.69 ng/mL, with an IC50 of 0. 438 1 ng/mL and it was about 3 times more sensitive than that of colorimetric ELISA. It has been validated on cereals samples in terms of precision （ intra- and interassay coefficient variations of less than 9. 1 % and 14. 7 % , respectively） and accuracy （mean recovery 79.6 % -97.6 % ）. These assay performances indicated that CLEIA was capable of being applied for the specific detection and routine monitoring of ZEN in corn samples.关键词
玉米赤霉烯酮（ZEN/间接竞争化学发光酶免疫法（CLEIA/检测Key words
zearalenone （ZEN）/chemiluminescence enzyme immunoassay（CLEIA）/determination分类
化学化工引用本文复制引用
卢江,许杨,楚金申,谢琳..化学发光酶免疫法检测玉米中玉米赤霉烯酮[J].食品与发酵工业,2011,37(5):160-165,6.基金项目
国家“863”计划项目 （）
