食品科学2011，Vol.32Issue(15)：153-157,5.
扩展青霉拮抗菌的筛选鉴定及抗菌物质分析
A Bacillus spp.Strain against Penicillium expansum： Screening,Identification,Phylogenetic Analysis and Activity of Extracellular Antibacterial Substances from It
摘要
Abstract
In order to obtain a Bacillus spp.that can protect post-harvest fruits from blue mould and patulin,plate confrontation test and double layer agar diffusion method were used for strain screening in this study.The Bacillus strain LPL-T12 isolated from oolong tea exhibited the best antibacterial effect,whose fermentation supernatant had an inhibitory zone diameter of（22.74 ± 0.44） mm against Penicillium expansum 3.3703.In addition,LPL-T12 was identified as Bacillus amyloliquefaciens through morphological observation,physiological and biochemical experiments,16S rDNA sequence analysis and specific PCR amplification.After the fermentation supernatant of the strain was subjected to ammonium sulfate precipitation and dialysis against several different pore-size membranes,a thermosensitive protein with molecular weight of more than 8000 D was obtained based on antibacterial activity analysis,protease hydrolysis and heating treatment.关键词
扩展青霉/芽孢杆菌/筛选/鉴定/抑菌物质Key words
Penicillium expansum/Bacillus spp./screening/identification/antibacterial substance分类
轻工纺织引用本文复制引用
孔维嘉,王洋,尚楠,张宝,李平兰..扩展青霉拮抗菌的筛选鉴定及抗菌物质分析[J].食品科学,2011,32(15):153-157,5.基金项目
国家自然科学基金项目 （）
国家“863”计划项目 （）
