食品科学2011，Vol.32Issue(15)：265-268,4.
马齿苋乙醇及其正乙醇提取物对D-半乳糖致衰老小鼠的学习记忆作用
Effects of Ethanol and n-Butanol-soluble Extract from Herba Portulacae on Learning and Memory Performance in D-Galactose-induced Senile Mice
金英子 1李迎军 2张红英2
作者信息
- 1. 延边大学医学部实验动物科,吉林延吉133002
- 2. 延边大学医学部基础医学院机能学实验中心,吉林延吉133002
- 折叠
摘要
Abstract
Objective： To study the effects and mechanisms of ethanol extract and its n-butanol-soluble fraction from Herba Portulacae on learning and memory performance in senile mice induced by D-galactose.Method： Mice were randomly divided into 6 groups： control group,model group and n-butanol-soluble fraction treatment（0.5 g/（kg·d） and 0.25 g/（kg·d）） groups and ethanol extract treatment groups（1 g/（kg·d） and 0.5 g/（kg·d）） groups.Normal saline was given to control group once daily for 42 consecutive days by subcutaneous injection in the neck back,and 1 g/（kg·d） D-galactose to the other 5 groups using the same administration route before intragastric administration of the same volume of distilled water and corresponding drugs（in aqueous solution） every afternoon during the administration period.Mouse learning and memory performance were tested by step-through test and water maze test,and MDA content and SOD and GSH-Px activities in the brain of senile mice were assayed.Results： Both dosages of n-butanol-soluble fraction could significantly improve mouse learning and memory performance（P 0.01）,and the dosage of 0.5 g/（kg·d） resulted in a significant increase in the activities of SOD and GSH-Px in the brain（P 0.05,P 0.01）,and the other dosage significantly decreased MDA content in the brain（P 0.05）.Meanwhile,ethanol extract from Herba Portulacae had a highly significant improving effect on mouse learning and memory performance（P 0.01） and significantly reduced MDA content in the brain（P 0.05）.Conclusion： From these results,we concluded that ethanol extract and its n-butanol-soluble fraction from Herba Portulacae are capable of significantly improving senile mouse learning and memory performance.The possible mechanisms may be through increasing SOD and GSH-Px activities and decreasing MDA content in the brain.Furthermore,ethanol extract from Herba Portulacae is superior to its n-butanol-soluble fraction.关键词
马齿苋/学习记忆/丙二醛/超氧化物歧化酶/谷胱甘肽过氧化物酶/衰老小鼠Key words
Herba Portulacae/learning and memory/MDA/SOD/GSH-Px/senile mouse分类
医药卫生引用本文复制引用
金英子,李迎军,张红英..马齿苋乙醇及其正乙醇提取物对D-半乳糖致衰老小鼠的学习记忆作用[J].食品科学,2011,32(15):265-268,4.
