食品科学2011，Vol.32Issue(15)：84-87,4.
仿刺参纵肌多糖的纯化及体外抗氧化
Purification and Antioxidant Activity in vitro of Polysaccharide from Apostichopus japonicus Longitudinal Muscle
摘要
Abstract
Objective： To separate and purify an antioxidant polysaccharide fraction from Apostichopus japonicus longitudinal muscle and to measure the abilities of the fraction and its sulfated counterpart to scavenge hydroxyl and superoxide anion free radicals.Methods： The separation and purification procedure was made up of papain hydrolysis,deprotenization with trichloroacetic acid,and Sephacryl S-400 gel filtration chromatography.The resulting fraction with higher free radical scavenging activity was selected for further studies including infrared spectral analysis and determination of molecular weight and sulfate content.Meanwhile,the fraction was sulfated,and its free radical scavenging activity was compared with that of its sulfated counterpart.Results： The obtained polysaccharide revealed an acidic glycosaminoglycan structure,whose relative molecular weight,sulfate content and substitution degree were 676 kD,10.98% and 0.285,respectively.Its sulfated counterpart had a sulfate content of 17.43% and a substitution degree of 0.360.Both of them had certain antioxidant activity to scavenge hydroxyl and superoxide anion free radicals,and the antioxidant activity of the sulfated polysaccharide was stronger.Conclusion： Sulfation can result in an increase in the sulfate content,substitution degree and antioxidant activity of the polysaccharide fraction with higher from Apostichopus japonicus longitudinal muscle.关键词
纵肌多糖/仿刺参/纯化/抗氧化Key words
longitudinal muscle polysaccharide/Apostichopus japonicus/purification/antioxidant activity分类
轻工纺织引用本文复制引用
张健,王茂剑,耿越,陈涛,赵云苹,刘京熙,高继庆..仿刺参纵肌多糖的纯化及体外抗氧化[J].食品科学,2011,32(15):84-87,4.基金项目
国家海洋局海洋公益性行业科研专项 （）
国家海洋局海洋经济规划科技推进平台与运作项目 （）
