食品工业科技Issue(8)：126-127,131,3.
佛手提取物及活性部位抑制血管紧张素转化酶活性的研究
Research on inhibition of extracts and active fraction in Citrus medica L.on angiotensin converting enzyme
摘要
Abstract
Objective：To screen the active fraction from Citrus medica L.with the inhibition to angiotensin converting enzyme（ACE）using different solvent.Methods：The various fractions were extracted with different solvent from Citrus medica L.According to the result of the inhibitory activity to ACE detected by HPLC,the fraction with the maximal inhibition was further purified with macro porous adsorption resin and eluted with different concentration of methanol.Results：Among the tested solvents,methanol extract showed the highest ACE inhibition,which was up to 77%.After purified with resin,the active fraction with maximal inhibition was obtained from 40% methanol.Conclusion：It was feasible to obtain the active fraction from Citrus medica L.with the inhibition to ACE by extracted with methanol,purified with macro porous adsorption resin and eluted with 40% of methanol.关键词
佛手/血管紧张素转化酶/高效液相色谱法/活性部位Key words
Citrus medica L./angiotensin converting enzyme/high performance liquid chromatography/active fraction分类
轻工纺织引用本文复制引用
常雯,李学刚,张化金,孙胜亮,叶小利..佛手提取物及活性部位抑制血管紧张素转化酶活性的研究[J].食品工业科技,2011,(8):126-127,131,3.基金项目
重庆市科委“十一五”新药创制重大专项 （）
西南大学自然科学青年基金项目 （）
