食品工业科技Issue(8)：400-403,4.
高效液相色谱法同时测定饮料中十种食用合成着色剂
Determination of ten synthetic colorant in beverage products by high performance liquid chromatography with variable wavelength detector
摘要
Abstract
An analysis method for the determination of synthetic colorant residue in beverage products by high performance liquid chromatography-Variable Wavelength Detector was developed.Under acid condition,samples were adsorpted for 30 minutes by polyamide powder in 60℃ and then desorpted by alkaline ethanol-ammonia-water（volume ratio 70：1：29）under alkaline condition.The residues were determined by high performance liquid chromatography with Variable Wavelength Detector by external standard method.The results showed that the linear correlation of residue solvents was good in the range of 0.1 ~ 10.0μg/mL and the linear correlation coefficient was in the range of 0.9981 ~ 0.9998.When 0.5 ~ 10.0μg/mL samples were added,the average recoveries were 76.6% ~ 108% and the intra-and inter-day rates were in the range of 1.0% ~ 7.5% and 2.1% ~ 12.0% respectively.The detection limit of Tartrazine（E102）,Amaranth,Ponceau 4R,Sunset yellow,Allura acid AC（E129）,Fast green FCF,Brilliant blue and Auramine O were 0.1μg/mL,and Light green SF yellowish and Acid orange were 0.2μg/mL.This method was suitable for the analysis of synthetic colorants residue in beverage products.关键词
高效液相色谱/残留分析/合成着色剂/饮料Key words
high performance liquid chromatography（HPLC）/residues analysis/synthetic colorant/beverage分类
轻工纺织引用本文复制引用
郭平,金会会,李瑾瑾,郑燕,王远兴,李海燕..高效液相色谱法同时测定饮料中十种食用合成着色剂[J].食品工业科技,2011,(8):400-403,4.基金项目
食品科学与技术国家重点实验室自由探索资助课题 （）
出入境检验检疫行业标准制定计划 （）
