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蚕蛹蛋白酶解制备抗氧化肽的初步研究

李高扬 崔堂兵 陈亮

现代食品科技2011,Vol.27Issue(7):810-814,5.
现代食品科技2011,Vol.27Issue(7):810-814,5.

蚕蛹蛋白酶解制备抗氧化肽的初步研究

Preliminary Study of Enzymatic Hydrolysis of Silkworm Pupa Protein for Production ofAntioxidant peptide

李高扬 1崔堂兵 1陈亮1

作者信息

  • 1. 华南理工大学生物科学与工程学院,广东广州,510006
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摘要

Abstract

Silkworm pupa was crushed and defatted by acetone, giving silkworm pupa powder. Then the powder was hydrolyzed with five proteases (Alkali protease, neutral protease, pepsin, papain, trypsin). Among the tested enzymes, pepsin was confirmed as an optimal enzyme preparation to prepare silkworm pupa polypeptide in terms of DPPH scavenging activity of hydrolysates. The enzymatic hydrolysis procedure was op timized using orthogonal array method and the corresponding product was subjected to further analysis of antioxidant activity, such as reducing power and O2, OH and DPPH·scavenging activities.

关键词

蚕蛹蛋白/酶解/抗氧化/

Key words

silkworm pupa protein/ enzyme hydrolysis/ antioxidation/ peptide

引用本文复制引用

李高扬,崔堂兵,陈亮..蚕蛹蛋白酶解制备抗氧化肽的初步研究[J].现代食品科技,2011,27(7):810-814,5.

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