现代食品科技2011，Vol.27Issue(7)：731-733,801,4.
裂褶菌产内切β-1,3-葡聚糖酶的特性研究
Properties of endo-β-l,3-glucanase from Schizophyllum commune Fr.
摘要
Abstract
The endo-β-1,3-glucanase from schizophyllum commune Fr. was separated and purified effectively, the purity was identified by electrophoresis and then the enzymological properties were discussed. It showed that after purified by DEAE-Sephadex A-50 ion exchange chromatography and Sephadex G-75 gel filtration, pure endo-β-l,3-glucanase with molecular weight of 45KD could be obtained, and its optimum temperature and pH were 45 ℃ and 5.0 respectively; Zn2+,K+,Ag+,Hg2+ and Ca2+had an inhibitory effect on the enzyme activity while Cu2+, Fe2+and Ba2+ stimulated the activity; Michaelis constant(Km) of the enzyme was 0.8813 mg/mL. Its secondary structure analyzed by circular dichroism showed that the percentage of a-helix, fl-sheet, mm and random coil were 4.6%, 49.1%, 8.7% and 37.5% respectively, which represented the typical structure of β-glueanase.关键词
内切β-1,3-葡聚糖酶/纯化/鉴定/酶学性质Key words
endo-β-1,3- glucanase/ purification/ identify/ enzymological properties引用本文复制引用
郑必胜,周萌..裂褶菌产内切β-1,3-葡聚糖酶的特性研究[J].现代食品科技,2011,27(7):731-733,801,4.基金项目
广东省教育部产学研合作项目(2008B090500190) （2008B090500190）
