中国食品学报2011，Vol.11Issue(4)：62-70,9.
酸枣仁黄酮组分提取纯化工艺的研究
Study on Extraction and Purification Technology of Flavones from Zizyphi Spinosi Semen
摘要
Abstract
To obtain the best appropriate method and optimum conditions of extraction and purification of three flavones from Zizyphi spinosi semen(ZSS), the conditions of reflux and circulation ultrasonic extraction were optimized by orthogonal design respectively. Mitsubishi SP825 resin was selected from 7 different types of macroporous adsorption resins and the elution conditions of chromatography experiment were optimized. Experimental results showed that circulation ultrasonic extraction was better method than reflux extraction, and its optimized extraction conditions were as fol-lows: 70% ethanol solution, ratio of liquid to solid 20:1(mL/g), ultrasonic power 500 W, 2.0 h, 40 ℃. SP825 resin was tested with the highest adsorption capacity and yield. In chromatographic experiment using column packed with SP825 resin, Flavones A, spinosin and 6"'-feruloylspinosin could be eluted entirely with gradient elution mode of 35%, 45% and 55% ethanol solution.关键词
酸枣仁/黄酮/超声提取/大孔树脂Key words
zizyphi spinosi semen/ flavones/ ultrasonic exrtration/ macroporous resin引用本文复制引用
丁轲,谷禹,陆晶晶,伍军..酸枣仁黄酮组分提取纯化工艺的研究[J].中国食品学报,2011,11(4):62-70,9.基金项目
北京市优秀人才培养资助项目(No.20081D0502100229) （No.20081D0502100229）
农产品加工及贮藏工程北京市重点建设学科(No.PXM2009_014207_078172) （No.PXM2009_014207_078172）
