中国食品学报2011，Vol.11Issue(4)：42-47,6.
转谷氨酰胺酶交联酶晶体的制备及其结构表征
Preparation of Cross-linked Transglutaminase (MTG) Crystals and its Structure Characterization
摘要
Abstract
Transglutaminase (MTG) crystals was cross-linked chemically by bifunctional reagent-glutaric dialde-hyde, the cross-linking conditions were obtained, and the cross-linked enzyme was scanned by scanning electronic microscope and its structure was characterized by infrared spectrum. Cross-linking conditions were as follows, glutaric di-aldehyde 1%, pH 6, 4℃ for 40 min. The scanning picture showed that when the free MTG cross-linked into crystals, the space molecular structure changed, the size of the cross-linked crystals were uniform and anomalous polyhedron. The infrared spectrum showed that, strong peak appeared on 1 651 cm-1 in free MTG, while the strong peak appeared on 1 651 cm-1 in cross-linked crystals, peaks appeared on 1 281 cm-1 1241 cm-1 in cross-linked MTG, respectively, while there was no peak in alpha helix region.关键词
转谷氨酰胺酶/交联/酶晶体：表征Key words
transglutaminase (MTG)/ cross-link/ crystals/ characterization引用本文复制引用
韩春然,张娜,王强,张毅方,刘颖,马永强..转谷氨酰胺酶交联酶晶体的制备及其结构表征[J].中国食品学报,2011,11(4):42-47,6.基金项目
国家高技术研究发展计划(863计划,2008AA10Z303) （863计划,2008AA10Z303）
黑龙江省高校科技创新团队建设计划(No.2010td04)资助 （No.2010td04）
