N-马来酰-L-缬氨酸酯姜黄素诱导胃癌MGC-803细胞凋亡的机制OA北大核心CSCDCSTPCD
Mechanisms of MVC-induced MGC-803 Cell Apoptosis
目的 研究N-来酰-L-缬氨酸酯姜黄素(MVC)诱导人胃癌MGC-803细胞凋亡的分子机制.方法 采用MTT法检测MVC对肿瘤细胞的生长抑制作用；Hoechst 33342/PI核荧光双染色法检测细胞凋亡的改变；运用Western blot技术检测参与细胞凋亡相关基因的蛋白表达.结果 20 μmol/L MVC可明显抑制MGC-803细胞生长,48 h IC50值为12.6 μmol/L;20μmol/L MVC可诱导MGC-803细胞凋亡；MV…查看全部>>
Objective To study on the mechanisms of MVC-induced MGC-803 cell apoptosis. Methods The role of MVC on cell growth was tested by MTT in vitro. Apoptotic cells were detected using Hoechst Hoechst 33342/PI staining. The level of the protein involved in apoptosis metabolism was detected by Western blot. Results MCV markedly inhibidited MGC-803 cell growth and IC50 of the 48 hour was 12. 6 μmol/L. Apoptosis was observed with in 48 hour after 20 μmol/L MCV tr…查看全部>>
孟爱国;刘春艳
063000河北唐山,河北联合大学附属医院检验科河北联合大学药学院中药学科
医药卫生
N-马来酰-L-缬氨酸酯姜黄素细胞凋亡MGC-803细胞
MVC Cells apoptosis MGC-803 cells
《肿瘤防治研究》 2011 (9)
995-997,3
河北省自然科学基金资助项目(C2010001758)
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