食品工业科技2011，Vol.32Issue(7)：178-181,4.
微小毛霉凝乳酶基因在毕赤酵母中的诱导表达研究
Study on induced expression of the rennin gene of Mucor pusillus by Pichia pastoris
摘要
Abstract
Objectives:To study the growth,enzyme-producing properties of the yeast and total protein of cultures during the induced expression of Mucor pusillus rennin in recombinant Pichia pastoris GS115PJ5.Method:Growth curve,milk-clotting activity,proteolytic activity,total protein content were determined.Results:The results showed that the yeast entered the stationary phase after incubation for 24h, and started to enter the decline phase after 240h.A strong band at about 47000 was shown by SDS-PAGE.Analysis of enzymatic activity showed that rennin accumulated rapidly after incubation for 144h and reached the maximum milk-clotting activity(300SU/mL)at 192h. Proteolytic activity,the ratio of milk-clotting activity and proteolytic activity (C/P) and total protein content were determined to be 10.75U/mL,27.9,0.189mg/mL,respectively.Conclusion: Mucor pusillus rennin was expressed effectively in Pichia pastoris.关键词
微小毛霉凝乳酶/巴斯德毕赤酵母/表达Key words
Mucor pusillus rennin/Pichia pastoris/expression分类
生物科学引用本文复制引用
郑丽,王昕,王景会,杨贞耐..微小毛霉凝乳酶基因在毕赤酵母中的诱导表达研究[J].食品工业科技,2011,32(7):178-181,4.基金项目
国家高技术研究发展计划(863计划)(2006AA10Z306) （863计划）
农业部现代农业产业技术体系建设专项资金资助项目(nycytx-0502). （nycytx-0502）
