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高产L-乳酸干酪乳杆菌的选育及发酵条件研究

秦浩 张伟国 葛向阳 康传利

食品工业科技2011,Vol.32Issue(7):223-225,245,4.
食品工业科技2011,Vol.32Issue(7):223-225,245,4.

高产L-乳酸干酪乳杆菌的选育及发酵条件研究

Study on screening the high L-lactic acid production strain of Lactobacillus casei and its fermentation conditions

秦浩 1张伟国 1葛向阳 1康传利1

作者信息

  • 1. 江南大学工业生物技术教育部重点实验室,江苏无锡,214122
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摘要

Abstract

Using Lactobacillus casei G-02 as a starting strain for induction by diethylsulfate( DES),N-methyl-N'-nitro-soguanidine( NTG) treatment, and selecting using high temperature and high sugar concentration culture conditions,a L-lactic acid hyper-producer G-04 was obtained.The effect of dissolved oxygen,neutralizer, and feeding on L-lactic acid production in 7L bioreactor were also discussed. Experimental results showed that the strain can accumulate L-lactic acid 188g/L, sugar conversion efficiency more than 90% and the fermentation period less than 44h under the optimized fermentation conditions.

关键词

L-乳酸/菌种选育/干酪乳杆菌/发酵条件

Key words

L-lactic acid/breeding/Lactobacillus casei/fermentation condition

分类

轻工纺织

引用本文复制引用

秦浩,张伟国,葛向阳,康传利..高产L-乳酸干酪乳杆菌的选育及发酵条件研究[J].食品工业科技,2011,32(7):223-225,245,4.

食品工业科技

OA北大核心CSCDCSTPCD

1002-0306

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