食品工业科技2011，Vol.32Issue(7)：260-262,266,4.
大孔吸附树脂分离纯化花椒总黄酮研究
Study on separation and purification of flavonoids in Zanthoxylum bungeanum by macroporous adsorption resin
摘要
Abstract
Adsorption and desorption characteristics of macroporous adsorption resins D4006, NKA-9, X-5, HPD450,HPD600 and AB-8 toward flavones of Zanthoxylum bungeanum were investigated.The best macroporous adsorption resin was chosen to carry out dynamic adsorption experiments, while the purification process was acquired.The results showed that the AB-8 macroporous resin gave the best effect of separation with concent ratio 3.0~4.0mg/mL,the feed solution pH 4.5 and the sampling rate 2.5mL/min, respectively, and the concent ratio of elution was 70% alcohol, with eluant volume 3BV(bed volume).By this technology the flavonoid content was increased from 51.46% of the crude extract to 90.25% of the purified product and the yield was 81.65%.High performance liquid chromatography (HPLC) analysis result showed that the purity of obtained flavonoids was significantly increased,and the rutin content was higher than other flavonoids.关键词
花椒/黄酮/大孔吸附树脂/纯化Key words
Zanthoxylum bungeanum/flavonoid/macroporous resin/purification分类
轻工纺织引用本文复制引用
吴亮亮,石雪萍,张卫明..大孔吸附树脂分离纯化花椒总黄酮研究[J].食品工业科技,2011,32(7):260-262,266,4.基金项目
国家"十一五"科技支撑计划重点课题(2006BAD06B02). （2006BAD06B02）
