食品工业科技2011，Vol.32Issue(7)：403-406,4.
海参多糖硫酸根含量的离子色谱法测定及其差异分析
Determination of sulfate content of sea cucumber polysaccharides by ion chromatography and analysis of the differences
摘要
Abstract
Sulfate in sea cucumber polysaccharide (crude sample) ,sea cucumber chondroitin sulfate( SC-CHS) and sea cucumber fucan (SC-FUC) were hydrolyzed by TFA, separated and determinated by ICS-2000 system. The average recovery was 100.90% ,RSD =2.12% ,the detection limitation reached 3.9×10-2 μg/mL.Compared with the traditional determination method of sulf ate, this method was reproducible, much simple and more accurate.The method applied to quantitative determination of sulfate in other suifated polysaccharides.The results showed that sulfate in all kinds of SC-CHS had a higher content than in SC-FUC,characteristical differences of sulfate content existed among different kinds of sea sucumber polysaccharide.关键词
离子色谱/硫酸根/海参多糖/海参硫酸软骨素/海参岩藻聚糖硫酸酯Key words
ion chromatography/sulfate/sea cucumber polysaccharides/sea cucumber chondroitin sulfate/sea cucumber fucan分类
轻工纺织引用本文复制引用
李国云,尹利昂,陈士国,王玉明,高瑞昌,徐杰,薛长湖..海参多糖硫酸根含量的离子色谱法测定及其差异分析[J].食品工业科技,2011,32(7):403-406,4.基金项目
国家高技术研究发展计划资助项目(2007AA091805). （2007AA091805）
