食品工业科技2011，Vol.32Issue(7)：420-422,3.
反相高效液相色谱法测定紫山药中薯蓣皂苷的含量
Determination of diosein in purple Dioscoroa alata Linn.by RP-HPLG
摘要
Abstract
To develop a RP-HPLC method for the determination of dioscin in purple Dioscorea alata Linn.After being extracted by refluxing with 70% MeOH for 1 hour, samples were achieved and separated by DiamonsilTM C18 column (250mm×4.6mm,5pm) with methanol-water (84:l6) as mobile phase.The flow rate was 1mL·min-1.The column temperature was 30℃.The UV detector was set at 206nm.Dioscin was well-separated.The linear calibration curve was obtained in the concentration range of 0.65~65μg·Ml-1 (r = 0.9999), the average recovery of dioscin was 100.6%.The contents of dioscin in purple Dioscorea alata Linn.of different habitats was in larger difference.The method was simple,accurate and reproducible.lt was also proposed to be used for the quality control of dioscin in Dioscorea opposite Thunb and Dioscorea alata Linn.关键词
薯蓣皂苷/紫山药/山药/RP-HPLCKey words
dioscin/purple Dioscorea alata linn/Dioscorea opposite Thunb/RP-HPLC分类
轻工纺织引用本文复制引用
周新勇,宋曙辉,罗晖,张丽梅..反相高效液相色谱法测定紫山药中薯蓣皂苷的含量[J].食品工业科技,2011,32(7):420-422,3.基金项目
国家科技支撑计划课题(2008BAD91B01). （2008BAD91B01）
