中国食品学报2011，Vol.11Issue(5)：108-113,6.
毕赤酵母表达重组人源Cu/ZnSOD的分离纯化与稳定性表征
Purification of Recombinant Human Cu/Zn SOD Expressed in Pichia Yeast and Its Stability Characterization
摘要
Abstract
Conventional methods for purification, including alcohol sedimentation, Strong cation exchange and strong anion exchange, were carried out to purify SOD, which were expressed by Pichia yeast system. Then the sensitive to temperature and pH condition of purified SOD were determined. Data showed that large quantity of SOD proteins, which were detected for only one peak by reversed phase chromatography, can be obtained by these methods. The activity of these proteins can reserved more than 85%, while they were incubated in the condition of under 75 ℃ or during pH 3~ 10. It is obviously that Pichia yeast system can produce large quantities of SOD proteins with low cost and increase the stability of SOD, which are in favour of industrial manufacture and SOD applications.关键词
毕赤酵母/Cu/Zn SOD/纯化/稳定性Key words
Pichia yeast/Cu/Zn SOD/purify/stability引用本文复制引用
杨志强,张晨,敖伟,蔡泽恩,刘树滔,饶平凡..毕赤酵母表达重组人源Cu/ZnSOD的分离纯化与稳定性表征[J].中国食品学报,2011,11(5):108-113,6.基金项目
国家自然科学基金项目(No.31071497) （No.31071497）
福建省卫教联合攻关项目(WKJ2008-2-47) （WKJ2008-2-47）
福建省高等学校新世纪优秀人才支持计划资助项目(SW2006-15) （SW2006-15）
