食品与机械2011，Vol.27Issue(4)：7-9,24,4.DOI:10.3696/j.issn.1003-5788.2011.04.002
转谷氨酰胺酶活性基团研究
Determination of transglutaminase activity groups
摘要
Abstract
The transglutaminase were selectively modified by ten chemical reagents such as PMSF, NBS, Iac, BrAc, SUAN, NEM, DTT, Ne,Soj, EDC, WRK, respectively. It can be found that (he enzyme activity was decreased significantly after modification of PMSF, NBS. Iac, BrAc, SUAN, NEM, but oppositely the DTT, Na2SOj > EDC and WRK didn't obviously affect the transglutaminase etuyme activity. Study of the chemical modification demonstrated that the Serine residues, Tryplophan residues. Histidine residues, Lysine residues and Cysteinyl residues were essential functional groups inside of the transglutamin?se active site, at the same time suggested disulfide bond and Glutamate( Aspartate) residues didn't significantly affect the enzyme activity and are not located in the en-zyme active site.关键词
转谷氨酰胺酶/化学修饰/活性中心Key words
tranaglutaminase/ chemical modification/ active site引用本文复制引用
马永强,岳文静,张娜,张毅方,钱镭,刘颖..转谷氨酰胺酶活性基团研究[J].食品与机械,2011,27(4):7-9,24,4.基金项目
国家“863”计划项目(编号:2008AA10Z303) （编号:2008AA10Z303）
