食品与机械2011，Vol.27Issue(4)：90-93,4.DOI:10.3969/j.issn.1003-5788.2011.04.025
酸性染料比色法定量检测荷叶总生物碱
Definite determination of total alkaloids in lotus by acid dye colorimetry
摘要
Abstract
Several factors were studied including extraction time and standing time, pH value and dosage of the potassium acid phthalale buffer solution, volume of bromcresol green and so on factors by u-sing uniform design method. The result showed that the maximum absorptive wavelength of total alkaloids of Lotus leaf was 416 nm by acid dye colorimetry, extracting time was 60 s, standing time was 150 min. And the optimum conditions of definite determination of total alkaloids in Lotus were: pH value of buffer solution 2.0. Volume of buffer solution 0. 5 mL. Dosage of bromcresol green 1. 0 mL Simultaneously, the regression equation y = 14. 12x - 0.013,R2 = 0. 999 8 (n = 5) , average recovery rate 98. 35 % , RSD 2. 11%. The method has good linear relationship, reproducibility, and popularization and application. And the method was much easier, faster and more accurate.关键词
总生物碱/酸性染料比色法/荷叶/检测Key words
total alkaloids/acid dye colorimetry/ lotus leaf/detection引用本文复制引用
邓燕莉,罗金波,黄甜,张玥,肖文军..酸性染料比色法定量检测荷叶总生物碱[J].食品与机械,2011,27(4):90-93,4.基金项目
国家科技部APEC基金项目(编号:200602) （编号:200602）
湖南省科技重点项目(编号:2005WK2002) （编号:2005WK2002）
