现代食品科技2011，Vol.27Issue(9)：1081-1083,1095,4.
磁性壳聚糖固定化转谷氨酰胺酶的研究
Immobilization of Transglutaminase on Magnetic Chitosan Microsphere
摘要
Abstract
Immobilization and enzymological properties of transglutaminase immobilized by a chitosan compound with Fe3O4 as a carrier were investigated in this study. Magnetic chitosan microspheres were prepared by reverse phase suspension process using Fe3O4 as core, paraffin as dispersed medium, Span-80 as emulsifier and epichlorohydrin as cross Unking agent. The carrier had well-shaped spherical form with smooth surface, and its mean particle size was 5.0 urn. The immobilization of transglutaminase onto magnetic chitosan microspheres by cross-linking with glutaraldehyde was investigated. The optimal immobilization conditions for transglutaminase were: transglutaminase linked with carrier for 1 hour after reacted with 1.5% (V/V) glutaraldehyde for 5 hours in buffer at pH 6.0. Under the optimum conditions, the activity recovery was 52.4%. Moreover, the optimal reaction temperature of immobilized transglutaminase was 50 °C, being 5 'C higher than that of free transglutaminase. The optimal pH of immobilzed transglutaminase was 8. The immobilized enzyme showed higher thermal stability than the free enzymes.关键词
磁性壳聚糖/固定化/转谷氨酰胺酶Key words
magnetic chitosan/ immobilization/ transglutaminase引用本文复制引用
刘颖,王鑫,岳文静,王蕊,马永强..磁性壳聚糖固定化转谷氨酰胺酶的研究[J].现代食品科技,2011,27(9):1081-1083,1095,4.基金项目
国家高新技术研究发展863计划(2008M102303) （2008M102303）
